REPOZYTORIUM
UNIWERSYTETU MIKOŁAJA KOPERNIKA
W TORUNIU
jest repozytorium instytucjonalnym Uniwersytetu Mikołaja Kopernika w Toruniu. Zadaniem Repozytorium jest promowanie dorobku naukowego i badań prowadzonych na UMK oraz wspomaganie dydaktyki.
Najnowsze publikacje
Development of mixed-mode dispersive solid phase extraction of antisense oligonucleotides using commercial adsorbents
(Elsevier, 2026-06) Studzińska, Sylwia; Ostrowska, Karolina
Sample preparation remains a challenge in the bioanalysis of antisense oligonucleotides due to their polyanionic character, structural complexity, and interactions with proteins. In the current study, a novel mixed-mode extraction methodology for oligonucleotides was developed utilizing commercially available sorbents and subsequently applied to serum matrices. Notably, this work constitutes the first evidence that broadly available adsorbents, not specifically designed for oligonucleotide isolation, can nevertheless be effectively employed for their extraction. Five sorbents with various functional groups were systematically evaluated to elucidate their interaction with antisense oligonucleotides. As a result, dispersive solid phase extraction procedures were developed, with adsorption and desorption controlled by electrostatic, hydrogen-bonding, π-π, and hydrophobic interactions. Elution conditions were optimized using a central composite design, demonstrating that efficient desorption requires simultaneous modulation of salt pH and concentration, and organic solvent content. The results showed that extraction proceeds via a mixed mode mechanism. Two silica-based sorbents modified with phenylboronic acid and 2 (2 pyridyl)ethyl groups exhibited the most favorable balance of recovery, reproducibility, and sorption capacity, achieving recoveries up to 93%. Kinetic studies revealed rapid adsorption for both sorbents, while differences in desorption influenced method transferability from dispersive solid phase extraction to solid phase extraction. Finally, the extraction procedure for phenylboronic acid-based material enabled one step isolation of antisense oligonucleotides from serum without prior protein removal. Although substantial (1:5 v/v) serum dilution was required and recoveries were moderate (59-71%), the approach represents an alternative to currently used methods. Moreover, it expands the bioanalytical tools for mixed mode oligonucleotide extraction using widely available commercial sorbents.
The Pierogi Problem: Cosmopolitan Appetites and the Reinvention of Polish Food_book revies
(Slavonic and East European Review, 2026-04-01) Goszczyński Wojciech
What does Polishness taste like? Who defines its flavour? How is it connected to Poland’s history and the global consumer movement? These questions are explored by a team of scholars working at the intersection of Polish (Bachórz, Halawa) and international (Parasecoli) food studies.
Intercity coopetition and regional innovation: The role of urban polycentricity
(Sage, 2026) Yang, Yuting; Lu, Jiayi; Derudder, Ben
Intercity coopetition—the dynamic interplay between cooperation and competition—is central to regional innovation, enabling knowledge exchange across cities. However, the spatial organization of these dynamics among functionally diverse cities and their implications for regional innovation outcomes remain underexplored. This study examines how urban polycentricity, captured through the balance of intercity coopetition relations, affects regional innovation performance. Methodologically, we develop an intercity coopetition index that incorporates both geographical overlap in collaboration networks and technological proximity. Empirically, we draw on longitudinal patent co-application data from nineteen Chinese urban regions between 1990 and 2022. Using fixed effect and instrumental variables models, we find that a balance in cooperation alone has a negative effect on regional innovation. In contrast, regional innovation performance is driven by the interaction between the strength of competitive ties and the balance of competition across cities. We conclude by discussing the policy implications of these findings for fostering spatially coordinated innovation systems.
Interconnected track and tech: the impact of multiplex transport and technology networks on labour market integration in the Yangtze River Delta, China
(Routledge, 2025-10) Liu, Xueqing; Derudder, Ben; Witlox, Frank
Despite growing scholarly attention to the role of urban networks in explaining regional integration dynamics, a more explicit focus on the multiplexity of infrastructure- and knowledge-based networks is required for understanding the nuanced ways in which cities regionally integrate. Applying a panel model to data for the Yangtze River Delta for the period 2014–2021, we juxtapose the impacts of transport and technology connections on labour market integration. To this end, we abstract both types of connections into a two-layer network and explore their (potential) interplays. Our analysis reveals that while transport networks show a positive correlation with labour market integration, technology networks demonstrate a complex U-shaped effect. We also find evidence for nuanced interactions in the multiplex network: (1) cities’ connectivity in both networks leads to a U-shaped effect on labour market integration due to the dominant role of technology connections; (2) cities’ interdependencies within the twolayer network lead to synergy effects, specifically manifested as increased integration of labour markets. We reflect on the broader implications of our findings for regional development strategies and discuss possible avenues for future research.
Polycentricity and technological diversification: divergent pathways across Chinese urban regions
(Taylor & Francis, 2026) Lu, Jiayi; Yang, Yuting; Cao, Zhan; Derudder, Ben
This paper examines how polycentricity shapes technological diversification in Chinese urban regions. Beyond morphological and functional dimensions, we introduce knowledge polycentricity, reflecting the balance of innovation across technological domains. Using patent data (2006–20), diversification is measured via relative relatedness density and estimated through fixed-effects and instrumental variable models. Findings highlight intra-regional functional polycentricity as the main driver, while other dimensions mainly act through interactions. Developmental heterogeneity produces distinct diversification pathways. Results call for innovation policies tailored to polycentricity characteristics and diversification strategies.