Ligand fishing using new chitosan based functionalized AndrogenReceptor magnetic particles

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dc.contributor.author Marszałł, Michał Piotr
dc.contributor.author Sroka, Wiktor
dc.contributor.author Sikora, Adam
dc.contributor.author Chełminiak-Dudkiewicz, Dorota
dc.contributor.author Ziegler-Borowska, Marta
dc.contributor.author Siódmiak, Tomasz
dc.contributor.author Moaddel, Ruin
dc.date.accessioned 2019-05-30T09:57:17Z
dc.date.available 2019-05-30T09:57:17Z
dc.date.issued 2016
dc.identifier.citation Journal of Pharmaceutical and Biomedical Analysis vol. 127, 2016, pp.129–135.
dc.identifier.issn 0731-7085
dc.identifier.other https://doi.org/10.1016/j.jpba.2016.04.013
dc.identifier.uri http://repozytorium.umk.pl/handle/item/5884
dc.description.abstract Superparamagnetic nanoparticles with chemically modified chitosan has been proposed as a potentialsupport for the immobilization of the androgen receptor (AR). The study involved comparison of differentAR carriers like commercially available magnetic beads coated with silica (BcMag) and chitosan coatednanoparticles with different amount of amino groups. The immobilization was carried out through cova-lent immobilization of the AR through the terminal amino group or through available carboxylic acids.The initial characterization of the AR coated magnetic beads was carried out with dihydrotestosterone,a known AR ligand. Subsequently, chitosan modified nanporticles with long-distanced primary aminogroups (Fe3O4CS-(NH2)3) (upto 8.34 mM/g) were used for further study to isolate known AR ligands(bicalutamide, flutamide, hydroxyflutamide and levonogestrel) from a mixture of tested compounds inammonium acetate buffer [10 mM, pH 7.4]. The results showed that the selected nanoparticles are apromising semi-quantitative tool for the identification of high affinity compounds to AR and might be ofspecial importance in the identification of novel agonists or antiandrogens.
dc.description.sponsorship National ScienceCentre: DEC-2011/03/D/NZ7/02296.
dc.language.iso eng
dc.publisher Elsevier
dc.rights CC0 1.0 Universal
dc.rights info:eu-repo/semantics/openAccess
dc.rights.uri http://creativecommons.org/publicdomain/zero/1.0/
dc.subject Androgen receptor
dc.subject Antiandrogenes
dc.subject Chitosan
dc.subject Ligand fishing
dc.subject Magnetic beads
dc.title Ligand fishing using new chitosan based functionalized AndrogenReceptor magnetic particles
dc.type info:eu-repo/semantics/article

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