Ligand fishing using new chitosan based functionalized AndrogenReceptor magnetic particles

dc.contributor.authorMarszałł, Michał Piotr
dc.contributor.authorSroka, Wiktor
dc.contributor.authorSikora, Adam
dc.contributor.authorChełminiak-Dudkiewicz, Dorota
dc.contributor.authorZiegler-Borowska, Marta
dc.contributor.authorSiódmiak, Tomasz
dc.contributor.authorMoaddel, Ruin
dc.date.accessioned2019-05-30T09:57:17Z
dc.date.available2019-05-30T09:57:17Z
dc.date.issued2016
dc.description.abstractSuperparamagnetic nanoparticles with chemically modified chitosan has been proposed as a potentialsupport for the immobilization of the androgen receptor (AR). The study involved comparison of differentAR carriers like commercially available magnetic beads coated with silica (BcMag) and chitosan coatednanoparticles with different amount of amino groups. The immobilization was carried out through cova-lent immobilization of the AR through the terminal amino group or through available carboxylic acids.The initial characterization of the AR coated magnetic beads was carried out with dihydrotestosterone,a known AR ligand. Subsequently, chitosan modified nanporticles with long-distanced primary aminogroups (Fe3O4CS-(NH2)3) (upto 8.34 mM/g) were used for further study to isolate known AR ligands(bicalutamide, flutamide, hydroxyflutamide and levonogestrel) from a mixture of tested compounds inammonium acetate buffer [10 mM, pH 7.4]. The results showed that the selected nanoparticles are apromising semi-quantitative tool for the identification of high affinity compounds to AR and might be ofspecial importance in the identification of novel agonists or antiandrogens.pl
dc.description.sponsorshipNational ScienceCentre: DEC-2011/03/D/NZ7/02296.pl
dc.identifier.citationJournal of Pharmaceutical and Biomedical Analysis vol. 127, 2016, pp.129–135.pl
dc.identifier.issn0731-7085
dc.identifier.otherhttps://doi.org/10.1016/j.jpba.2016.04.013
dc.identifier.urihttp://repozytorium.umk.pl/handle/item/5884
dc.language.isoengpl
dc.publisherElsevierpl
dc.rightsCC0 1.0 Universal*
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/publicdomain/zero/1.0/*
dc.subjectAndrogen receptorpl
dc.subjectAntiandrogenespl
dc.subjectChitosanpl
dc.subjectLigand fishingpl
dc.subjectMagnetic beadspl
dc.titleLigand fishing using new chitosan based functionalized AndrogenReceptor magnetic particlespl
dc.typeinfo:eu-repo/semantics/articlepl

Files

Original bundle

Loading...
Thumbnail Image
Name:
JPBA.pdf
Size:
1.02 MB
Format:
Adobe Portable Document Format

License bundle

Loading...
Thumbnail Image
Name:
license.txt
Size:
1.34 KB
Format:
Item-specific license agreed upon to submission
Description:

Collections