Enantioselective acetylation of (R,S)-atenolol: The use of Candidarugosa lipases immobilized onto magnetic chitosan nanoparticles inenzyme-catalyzed biotransformation

Abstract

tThis paper describes the enzyme immobilization protocol as well as the enzymatic method for the directresolution of (R,S)-atenolol. The used magnetic enzyme carriers possess on their surface new-synthetizedchitosan derivatives with free amine groups distanced by ethyl or butyl chain. Additionally the cat-alytic activity of two types of commercially available lipases from Candida rugosa immobilized onto twodifferent magnetic nanoparticles were compared. The highest values of enantioselectivity (E = 66.9), enan-tiomeric excess of product (eep= 94.1%) and conversion (c = 41.84%) were obtained by using lipase fromCandida rugosa OF immobilized onto Fe3O4-CS-EtNH2. The study confirmed that even after 5 reactioncycles the immobilized lipase maintain its high catalytic activity.