Biogenic silver nanoparticles: assessment of their cytotoxicity, genotoxicity and study of capping proteins

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dc.contributor.author Wypij, Magdalena
dc.contributor.author Jędrzejewski, Tomasz
dc.contributor.author Ostrowski, Maciej
dc.contributor.author Trzcińska, Joanna
dc.contributor.author Rai, Mahendra
dc.contributor.author Golińska, Patrycja
dc.date.accessioned 2022-10-19T07:32:15Z
dc.date.available 2022-10-19T07:32:15Z
dc.date.issued 2020-07-02
dc.identifier.citation Molecules, vol. 25, 2020, pp.1-19.
dc.identifier.issn 1420-3049
dc.identifier.other 10.3390/molecules25133022
dc.identifier.uri http://repozytorium.umk.pl/handle/item/6776
dc.description.abstract The development of nanotechnology in the last two decades has led to the use of silver nanoparticles (AgNPs) in various biomedical applications, including antimicrobial, anti-inflammatory, and anticancer therapies. However, the potential of the medical application of AgNPs depends on the safety of their use. In this work, we assessed the in vitro cytotoxicity and genotoxicity of silver nanoparticles and identified biomolecules covering AgNPs synthesized from actinobacterial strain SH11. The cytotoxicity of AgNPs against MCF-7 human breast cancer cell line and murine macrophage cell line RAW 264.7 was studied by MTT assay, cell LDH (lactate dehydrogenase) release, and the measurement of ROS (reactive oxygen species) level while genotoxicity in Salmonella typhimurium cells was testing using the Ames test. The in vitro analysis showed that the tested nanoparticles demonstrated dose-dependent cytotoxicity against RAW264.6 macrophages and MCF-7 breast cancer cells. Moreover, biosynthesizedAgNPsdid not show a mutagenic e ect of S. typhimurium. The analyses and identification of biomolecules present on the surface of silver nanoparticles showed that they were associated with proteins. The SDS-PAGE (sodium dodecyl sulfate–polyacrylamide gel electrophoresis) analysis revealed the presence of 34 and 43 kDa protein bands. The identification of proteins performed by using LC-MS/MS (liquid chromatography with tandem mass spectrometry) demonstrated their highest homology to bacterial porins. Capping biomolecules of natural origin may be involved in the synthesis process of AgNPs or may be responsible for their stabilization. Moreover, the presence of natural proteins on the surface of bionanoparticles eliminates the postproduction steps of capping which is necessary for chemical synthesis to obtain the stable nanostructures required for application in medicine.
dc.description.sponsorship This research and the APC were funded by National Science Centre (NCN), grant number 2016/23/N/NZ9/00247.
dc.language.iso eng
dc.publisher Multidisciplinary Digital Publishing Institute
dc.relation.ispartofseries 25 (13);3022
dc.relation.ispartofseries ;3022
dc.rights Attribution-NonCommercial-NoDerivs 3.0 Poland
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/3.0/pl/
dc.subject biosynthesis
dc.subject capping proteins
dc.subject cytotoxicity
dc.subject genotoxicity
dc.subject silver nanoparticles
dc.title Biogenic silver nanoparticles: assessment of their cytotoxicity, genotoxicity and study of capping proteins
dc.type info:eu-repo/semantics/article

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