Analysis of Relative Expression Level of VEGF (Vascular Endothelial Growth Factor), HIF-1α (Hypoxia Inducible Factor 1α) and CTGF (Connective Tissue Growth Factor) Genes in Chronic Glomerulonephritis (CGN) Patients

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dc.contributor.author Donderski, Rafał
dc.contributor.author Szczepanek, Joanna
dc.contributor.author Domagalski, Krzysztof
dc.contributor.author Tretyn, Andrzej
dc.contributor.author Korenkiewicz, Jadwiga
dc.contributor.author Marszalek, Andrzej
dc.contributor.author Szymański, Andrzej
dc.contributor.author Wolski, Zbigniew
dc.contributor.author Odrowąż-Sypniewska, Grażyna
dc.contributor.author Manitius, Jacek
dc.date.accessioned 2015-08-04T07:19:56Z
dc.date.available 2015-08-04T07:19:56Z
dc.date.issued 2014-03
dc.identifier.citation Kidney and Blood Pressure Research vol. 38, 2014, pp. 83-91.
dc.identifier.issn 1420-4096
dc.identifier.uri http://repozytorium.umk.pl/handle/item/2911
dc.description.abstract Background/Aims: Analysis of gene expression in renal tissue is considered to be a diagnostic tool predicting the clinical course of glomerulonephritis. The present study quantified the relative transcript levels of VEGF, CTGF and HIF-1α in renal tissue to establish their relationship with some clinical variables in patients suffering from chronic glomerulonephritis (CGN). Methods: 28 patients (6F and 22M, mean age 51.2±15.0) with CGN were enrolled. Type of CNG recognized by kidney biopsy (histopatological evaluation) was as follows: minimal change disease (MCD)-3pts, IgA nephropathy-5pts, FSGS-3pts, membranous nephropathy-4pts, mesangio-proliferative glomerulonephritis-3pts; MPGN-1pts, lupus nephritis-6pts, granulomatosis with polyangitis-2 pts; hypertensive nephropathy- 3pts. Renal tissue from 3 individuals with normal eGFR and histology was taken as control. Mean clinical follow-up of patients was 12 months after biopsy eGFR and daily urinary protein excretion (DPE) was assessed at the time of biopsy and then in 6 months intervals. Real-time PCR was used to determine relative gene expression. The housekeeping gene GAPDH was used as normalization control. Results: At the time of the biopsy relative expression of 3 analyzed genes was diminished in comparison to control. There were statistically significant differences in VEGF gene relative expression level in patients which varied according to eGFR and tendency in patients which varied according to DPE. HIF-alfa and CTGF gene showed only a tendency. Conclusions: Overexpression of the VEGF gene in subjects with DPE>3,5g may point to insufficient oxygen supply in renal tissue which may result in tubulointerstitial fibrosis with further functional renal impairment and decline of eGFR.
dc.language.iso eng
dc.publisher S. Karger A.G.
dc.rights Attribution-NonCommercial-NoDerivs 3.0 Poland
dc.rights info:eu-repo/semantics/openAccess
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/3.0/pl/
dc.subject Chronic kidney disease (CKD)
dc.subject chronic glomerulonephritis
dc.subject relative gene expression level
dc.subject tubulointerstitial compartment damage
dc.title Analysis of Relative Expression Level of VEGF (Vascular Endothelial Growth Factor), HIF-1α (Hypoxia Inducible Factor 1α) and CTGF (Connective Tissue Growth Factor) Genes in Chronic Glomerulonephritis (CGN) Patients
dc.type info:eu-repo/semantics/article

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