Features of immune status in different states of uric acid metabolism in female rats

Abstract

Background. Previously, we found a wide range of uric acid metabolism parameters and functional relationships of uricemia and uricosuria with the parameters of immunity in healthy female rats analyzed. It was found by canonical correlation analysis that both parameters of uric acid metabolism determine the immunity status of rats by 71%. The purpose of this study is to determine the features of immune status in rats with quantitatively and qualitatively different uric acid metabolism. Material and Methods. Experiment was performed on 60 healthy female Wistar rats 220-300 g. Among them 10 animals remained intact, using tap water from drinking ad libitum. The rats of others groups for 6 days administered through the tube various fluids. The serum and urine levels of the uric acid (uricase method) were determined. In the blood, the parameters of immun ity were determined. From thymus and spleen made smears-imprints for counting splenocytogram and thymocytogram. For them, as well as immunocytogram and leukocytogram of blood, Shannon’s entropy was calculated. Results. Screening of immune profiles of four quantitative and qualitative uric acid metabolism clusters revealed 6 functional-metabolic patterns, two of which are quasilinear (enhanc ing and suppressing), with immune extremes at moderately elevated uricemia, two U-shaped with immune optimum at moderately reduced uricemia levels, as well as a non-reactive pattern. Discriminant analysis revealed 14 parameters of immunity, the constellation of which four clusters of metabolism of uric acid clearly distinguish. Conclusion. Endogenous uric acid exerts a modulatory immunotropic effect in healthy female rats.