Synthesis of magnetic nanoparticles with surface modified with chitosan and poly(acrylic acid) blends for biomedical application

Abstract

In recent years, the synthesis and characterization of nanoparticles have been the focus of intensive research. This type of nanoparticles have been highly employed in chemistry and biomedical applications such as magnetic hyperthermia, catalysis, diagnostic agent, and especially for biomolecule immobilization. The properties of the magnetic materials depends on the stabilizer type, which affect on covering surface. In this study the synthesis of new type of chitosan (CS) and poly (acrylic acid) (PAA) coated nanoparticles by photopolymerization is presented (Fig. 1) [1-3]. Pure monomer of acrylic acid were subjected to photopolymerization, while the photopolymerization kinetics was followed by FTIR spectroscopy. The band corresponding to the C=C stretching vibrations has been selected for calculation of conversion degree of monomer. The structure of prepared magnetic material was processed by ATR-FTIR spectroscopy and XRD analysis. The morphology and size of the prepared nanoparticles were characterized by transmission electron microscopy. The amount of free amino groups on the surface of magnetic nanoparticles was estimated by the ninhydrin method. The activity, reusability, and the amount of immobilizer lipase were determined.

[1] Chełminiak D., Ziegler-Borowska M., Kaczmarek H., Mater. Lett. 164 (2016) 464-467. [2] Ziegler-Borowska M., Siódmiak T., Chełminiak D., et. al. Appl. Surf. Sci. 288 (2014) 641-648. [3] Ziegler-Borowska M., Chełminiak D., Siódmiak T., et. al. Mater. Lett. 132 (2014) 63-65.

Acknowledgements The project was supported by research grant: National Science Centre 2014/15/B/NZ7/00972.