Wydział Nauk Biologicznych i Weterynaryjnych / Faculty of Biological and Veterinary Scienceshttps://repozytorium.umk.pl:443/handle/item/32024-03-28T17:14:29Z2024-03-28T17:14:29ZMycogenic Copper Oxide Nanoparticles For Fungal Infection Management in Agricultural Crop PlantsIngle, PramodShende, SudhirHande, DilipRai, MahendraGolinska, PatrycjaGade, Anikethttps://repozytorium.umk.pl:443/handle/item/69892024-02-20T00:20:30Z2023-01-01T00:00:00ZMycogenic Copper Oxide Nanoparticles For Fungal Infection Management in Agricultural Crop Plants
Ingle, Pramod; Shende, Sudhir; Hande, Dilip; Rai, Mahendra; Golinska, Patrycja; Gade, Aniket
Aspergillus infections are one of the significant threats to crop plants such as peanuts, cottonseed, maize, tree nuts, food, and feed. The fungal members of this genus can cause up to 100% losses in fruit plants under favorable conditions. It is thus a necessity to control these phytopathogenic fungi by a renewable, non-hazardous technology. Phoma spp. are well known to synthesize antifungal metabolites like 4,8-dihydroxy-6-methoxy-3-methyl-3,4-dihydro-1H-isochromen-1-one, Viridicatol, tenuazonic acid, alternariol, alternariol monomethyl ether, phomafungin, etc. In the present work, copper oxide nanoparticles (CuONPs) were synthesized using an aqueous extract of Phoma glomerata (MTCC-2210). They were assessed for in vitro antifungal activity against Aspergillus flavus and Aspergillus niger. CuONPs showed absorption maxima at 630 nm and an average size of 119.6 nm, which were stable at a zeta potential of -23.5 mV, forming the face-centered cubic (FCC) shaped crystalline structure as elucidated by XRD. FTIR confirmed the presence of various secondary metabolites from fungal extracts in the NPs capping. Kirby-Bauer disc diffusion assay indicated the antifungal activity with an average zone of inhibition of 19 +3 mm and 23 +4 mm diameter for A. niger and A. flavus, respectively. The serial dilution method
2
showed the MIC of 180 and 220 μg/ml against A. niger and A. flavus, respectively. MIC values were significantly compared to standard fungicide mancozeb. Green synthesized CuONPs using Phoma extract indicated significant fungicidal activity against test pathogens and paved their way to be applied in the agriculture fields for crop applications as a novel substitute to chemical fungicides.
2023-01-01T00:00:00ZZabezpieczenie zabytków odkrytych w podziemiach klasztoru służące zachowaniu dziedzictwa lokalnego - folderDrozd-Lipińska, AlicjaMajorek, MagdalenaMucha, NataliaNowak, Marcinhttps://repozytorium.umk.pl:443/handle/item/69412023-11-15T00:20:30Z2023-01-01T00:00:00ZZabezpieczenie zabytków odkrytych w podziemiach klasztoru służące zachowaniu dziedzictwa lokalnego - folder
Drozd-Lipińska, Alicja; Majorek, Magdalena; Mucha, Natalia; Nowak, Marcin
2023-01-01T00:00:00ZZabezpieczenie zabytków odkrytych w podziemiach klasztoru służące zachowaniu dziedzictwa lokalnegoDrozd-Lipińska, AlicjaMucha, NataliaMajorek, MagdalenaNowak, Marcinhttps://repozytorium.umk.pl:443/handle/item/69402023-11-15T00:20:33Z2023-01-01T00:00:00ZZabezpieczenie zabytków odkrytych w podziemiach klasztoru służące zachowaniu dziedzictwa lokalnego
Drozd-Lipińska, Alicja; Mucha, Natalia; Majorek, Magdalena; Nowak, Marcin
Autorzy i tytuły poszczególnych plansz:
Plansza nr 1: Alicja Drozd-Lipińska, Natalia Mucha, Badania ratownicze w krypcie pod ołtarzem Matki Bożej Nieustającej Pomocy w Kościele Franciszkańskim p.w. Znalezienia Krzyża Świętego w Radziejowie;
Plansze nr 2 - 3: Alicja Drozd-Lipińska, Natalia Mucha, Dawni mieszkańcy Radziejowa w świetle wyników badan antropologicznych ludzkich szczątków kostnych z krypty Kościoła Znalezienia Krzyża Świętego w Radziejowie;
Plansza nr 4: Magdalena Majorek, Marcin Nowak, Konserwacja i digitalizacja zabytków pochodzących z krypty;
Plansza nr 5: Magdalena Majorek, Marcin Nowak, Pochowani w krypcie – trumny;
Plansza nr 6: Magdalena Majorek, Marcin Nowak, Dewocjonalia – ważny element nowożytnej obrzędowości pogrzebowej;
Plansza nr 7: Magdalena Majorek, W świecie barw – wyroby włókiennicze odnalezione w krypcie;
Plansza nr 8: Magdalena Majorek, Przed i po konserwacji - pończochy i fular;
Plansza nr 9: Magdalena Majorek, Przed i po konserwacji - nakrycia głowy i pasamony;
Plansza nr 10: Magdalena Majorek, Ornat, stuły, manipularze – po konserwacji.
2023-01-01T00:00:00ZImmunologiczne badania nad molekularnym podobieństwem sulfohydrolaz lizosomalnychBiałkowski, Karolhttps://repozytorium.umk.pl:443/handle/item/68212023-01-31T00:20:31Z2023-01-30T00:00:00ZImmunologiczne badania nad molekularnym podobieństwem sulfohydrolaz lizosomalnych
Białkowski, Karol
1. Opracowano ulepszone postępowanie prowadzące do szybkiego oczyszczenia arylosulfataz A (ASA) i B (ASB) narządów wołowych do stanu bliskiego jednorodności elektroforetycznej. Kolejne procedury chromatograficzne zostały tak dobrane, że wyeliminowana została konieczność czasochłonnego zagęszczania preparatów przez ultrafiltrację, podczas której występują znaczne straty aktywności.
2. Uzyskano specyficzne, poliklonalne przeciwciała: IgG „anty ASA” i IgG „anty B1 I”. Przeciwciała te wyodrębniono z surowicy i oczyszczono na kolumnie białko A–sefarozy, otrzymując preparaty o wysokim mianie.
3. Miareczkowania immunologiczne wykazały bardzo wysokie podobieństwo czterech molekularnych form arylosulfatazy B. Sugeruje to silnie, że nie są to odrębne izoenzymy jak się niekiedy uważa, a najprawdopodobniej konformery ładunkowe, powstałe w wyniku procesów potranslacyjnych.
4. Stosując technikę immunomiareczkowania wykazano bardzo duże podobieństwo ASB z wątroby wołu i szczura. Ludzka ASB różniła się znacznie od ASB wołowej. Arylosulfatazy B z wątroby gołębia i minoga wykazywały brak immunologicznego podobieństwa do ASB wołowej.
5. Arylosulfataza A wołowa i owcza są immunologicznie identyczne. Wieprzowa ASA różni się bardzo nieznacznie od ASA wołowej. Świadczy to o wysokim podobieństwie strukturalnym tych trzech enzymów. Arylosulfataza A łożyska ludzkiego wykazuje znaczną odrębność immunologiczną w stosunku do enzymu wołowego.
Praca magisterska wykonana w latach 1990-1992 pod kierunkiem doktora Antoniego Leźnickiego w Zakładzie Biochemii Instytutu Biologii UMK.
Obrona pracy - 15.06.1992
2023-01-30T00:00:00ZDual Effect of the Extract from the Fungus Coriolus Versicolor on Lipopolysaccharide-Induced Cytokine Production in RAW 264.7 Macrophages Depending on the Lipopolysaccharide ConcentrationJędrzejewski, TomaszSobocińska, JustynaPawlikowska, MałgorzataDzialuk, ArturWrotek, Sylwiahttps://repozytorium.umk.pl:443/handle/item/67772022-10-19T23:20:32Z2022-07-20T00:00:00ZDual Effect of the Extract from the Fungus Coriolus Versicolor on Lipopolysaccharide-Induced Cytokine Production in RAW 264.7 Macrophages Depending on the Lipopolysaccharide Concentration
Jędrzejewski, Tomasz; Sobocińska, Justyna; Pawlikowska, Małgorzata; Dzialuk, Artur; Wrotek, Sylwia
Purpose: Extract from the fungus Coriolus versicolor (CV) is classified as an immunological response modifier. Previously, we have shown that this extract induces interleukin 6 (IL-6)-related extension of lipopolysaccharide (LPS)-induced fever. This study investigated the effect of CV extract on the production of pro-inflammatory cytokines and the expression of components of signal transduction pathways leading to the secretion of cytokines from RAW 264.7 macrophages stimulated with different doses of LPS. Methods: RAW 264.7 cells were stimulated with CV extract alone or co-treated with CV extract and LPS. The level of IL-6 and
tumour necrosis factor α (TNF-α) in the culture media was measured using ELISA. Protein expression of Toll-like receptor (TLR) 4, phosphorylated IκB (p-IκB), CD14 glycoprotein and phospho-phosphatidylinositol 3-kinase (p-PI3K) was evaluated using Western blot. The effects of TLR4, nuclear factor κB (NF-κB) and p-PI3K on cytokine secretion were estimated using inhibitors: TAK-242, JSH-23 and Y294002.
Results: CV extract itself stimulates the secretion of IL-6 and TNF-α and increases the expression of TLR4, p-IκB and p-PI3K. The presence of CV extract during the treatment of cells with lower concentrations of LPS (10 and 100 ng/mL) increases the cytokine production. Co-stimulation of cells with CV extract and LPS at a higher dose (500 ng/mL) decreases the secretion of cytokines. This effect is related to the changes in the expression of TLR4, CD14 glycoprotein, p-IκB and p-PI3K.
Conclusion: This is the first report showing that the CV extract-induced production of cytokines is mediated by the PI3K signalling pathway. This extract acts antagonistically or additively with LPS on the production of IL-6 and TNF-α, depending on the LPS concentration. Our results are helpful for illustrating the mechanisms for the immunostimulatory effect of CV extract in inflammatory
processes.
2022-07-20T00:00:00ZBiogenic silver nanoparticles: assessment of their cytotoxicity, genotoxicity and study of capping proteinsWypij, MagdalenaJędrzejewski, TomaszOstrowski, MaciejTrzcińska, JoannaRai, MahendraGolińska, Patrycjahttps://repozytorium.umk.pl:443/handle/item/67762022-10-19T23:20:28Z2020-07-02T00:00:00ZBiogenic silver nanoparticles: assessment of their cytotoxicity, genotoxicity and study of capping proteins
Wypij, Magdalena; Jędrzejewski, Tomasz; Ostrowski, Maciej; Trzcińska, Joanna; Rai, Mahendra; Golińska, Patrycja
The development of nanotechnology in the last two decades has led to the use of silver
nanoparticles (AgNPs) in various biomedical applications, including antimicrobial, anti-inflammatory,
and anticancer therapies. However, the potential of the medical application of AgNPs depends on
the safety of their use. In this work, we assessed the in vitro cytotoxicity and genotoxicity of silver
nanoparticles and identified biomolecules covering AgNPs synthesized from actinobacterial strain
SH11. The cytotoxicity of AgNPs against MCF-7 human breast cancer cell line and murine macrophage
cell line RAW 264.7 was studied by MTT assay, cell LDH (lactate dehydrogenase) release, and the
measurement of ROS (reactive oxygen species) level while genotoxicity in Salmonella typhimurium
cells was testing using the Ames test. The in vitro analysis showed that the tested nanoparticles
demonstrated dose-dependent cytotoxicity against RAW264.6 macrophages and MCF-7 breast cancer
cells. Moreover, biosynthesizedAgNPsdid not show a mutagenic e ect of S. typhimurium. The analyses
and identification of biomolecules present on the surface of silver nanoparticles showed that they were
associated with proteins. The SDS-PAGE (sodium dodecyl sulfate–polyacrylamide gel electrophoresis)
analysis revealed the presence of 34 and 43 kDa protein bands. The identification of proteins performed
by using LC-MS/MS (liquid chromatography with tandem mass spectrometry) demonstrated their
highest homology to bacterial porins. Capping biomolecules of natural origin may be involved in the
synthesis process of AgNPs or may be responsible for their stabilization. Moreover, the presence of
natural proteins on the surface of bionanoparticles eliminates the postproduction steps of capping
which is necessary for chemical synthesis to obtain the stable nanostructures required for application
in medicine.
2020-07-02T00:00:00ZHalophyte–Endophyte Interactions: Linking Microbiome Community Distribution and Functionality to SalinityHrynkiewicz, KatarzynaBliss Ursula, Furtadohttps://repozytorium.umk.pl:443/handle/item/66922022-01-21T00:20:28Z2021-01-01T00:00:00ZHalophyte–Endophyte Interactions: Linking Microbiome Community Distribution and Functionality to Salinity
Hrynkiewicz, Katarzyna; Bliss Ursula, Furtado
Many plants are unable to adapt to rapid environmental changes (e.g., salinity, drought, or limited nutrients) and may acquire assistance from microbes that have the capacity to increase tolerance of host-plants in stress conditions. By having the right microbes, the plants are more resilient! Such microbes include endophytes that inhabit inner tissues of the plant without causing symptoms of disease in their host. However, this plant–endophytic association exists only when chemical equilibrium is maintained between both, therefore making this mutualistic interaction even more unique. Therefore it is interesting to decode the endophytic community composition in halophytes specifically in the most salt-tolerant halophyte species Salicornia europaea, and further determine the factors that could affect this association. Moreover, understanding the endophytes potential plant growth-promoting activities in association with host (S. europaea) and non-host plant (non-halophytes) are the focus of this chapter.
2021-01-01T00:00:00ZTillage intensity and herbicide application influence surface-active springtail (Collembola) communities in Romanian vineyardsUlrich, Wernerhttps://repozytorium.umk.pl:443/handle/item/66812021-12-24T00:20:28Z2020-01-01T00:00:00ZTillage intensity and herbicide application influence surface-active springtail (Collembola) communities in Romanian vineyards
Ulrich, Werner
Vineyards are important perennial, often intensively managed agroecosystems. In most vineyards, ground vegetation
is controlled by tillage and/or the application of broadband herbicides with scarcely known effects on
the soil fauna. We studied a total of 16 commercial vineyards in the Târnave wine region, a typical viticultural
region in Transylvania, Central Romania. As a model organism for surface-dwelling arthropods we examined the
response of springtail (Collembola) species richness and activity density to vineyard tillage practices, fertilization,
and herbicide application. A total of 24 species in 10 families were found in the studied vineyards. Principal
coordinates analysis (PCoA) identified three distinct springtail communities linked to tillage and fertilization
practice. Springtail species richness was positively associated with high tillage intensity (frequency: once or
twice a year). Springtail activity density in inter-rows was positively correlated with inter-row tillage and
herbicide application under grapevines. For the first time we could show that springtail species assemblages in
vineyards were characterized by high niche overlap regarding soil quality (organic matter, pH, CaCO3, P and K),
indicating similar resource utilization. We conclude that the positive influence of vineyard management and
disturbance on surface-dwelling springtail communities is possibly due to the exclusion of potential competitors
and predators, the stimulation of microorganisms and/or an increased nutrient input.
2020-01-01T00:00:00ZThe cytoplasmic transcripts of larch microsporocytes.Smolinski, Dariusz Janhttps://repozytorium.umk.pl:443/handle/item/65572021-04-20T10:54:07Z2021-04-20T00:00:00ZThe cytoplasmic transcripts of larch microsporocytes.
Smolinski, Dariusz Jan
2021-04-20T00:00:00ZTranscriptome profiling and environmental linkage to salinity across Salicornia europaea vegetationBliss, UrsulaNagy, IstvanAsp, TorbenTyburski, JarosławSkorupa, MonikaGołebiewski, MarcinHulisz, PiotrHrynkiewicz, Katarzynahttps://repozytorium.umk.pl:443/handle/item/62212020-01-07T06:45:59Z2019-01-01T00:00:00ZTranscriptome profiling and environmental linkage to salinity across Salicornia europaea vegetation
Bliss, Ursula; Nagy, Istvan; Asp, Torben; Tyburski, Jarosław; Skorupa, Monika; Gołebiewski, Marcin; Hulisz, Piotr; Hrynkiewicz, Katarzyna
Background: Salicornia europaea, a succulent obligatory halophyte is the most salt-tolerant plant species in the world. It survives salt concentrations of more than 1 M. Therefore, it is a suitable model plant to identify genes involved in salt tolerance mechanisms that can be used for the improvement of crops. The changes in a plant’s gene expression in response to abiotic stresses may depend on factors like soil conditions at the site, seasonality, etc. To date, experiments were performed to study the gene expression of S. europaea only under controlled
conditions. Conversely, the present study investigates the transcriptome and physicochemical parameters of S. europaea shoots and roots from two different types of saline ecosystems growing under natural conditions.
Results: The level of soil salinity was higher at the naturally saline site than at the anthropogenic saline site. The parameters such as ECe, Na+, Cl−, Ca+, SO4
2− and HCO3− of the soils and plant organs significantly varied according
to sites and seasons. We found that Na+ mainly accumulated in shoots, whereas K+ and Ca2+ levels were higher in roots throughout the growing period. Moreover, changes in S. europaea gene expression were more prominent in seasons, than sites and plant organs. The 30 differentially expressed genes included enzymes for synthesis of Sadenosyl methionine, CP47 of light-harvesting complex II, photosystem I proteins, Hsp70 gene, ATP-dependent Clp proteases, ribulose bisphosphate carboxylase/oxygenase (Rubisco), phenylalanine ammonia-lyase (PAL), cytochrome c oxidase (COX) and ATP synthase.
Conclusion: The comparisons made based on two seasons, plant organs and two different sites suggest the importance of seasonal variations in gene expression of S. europaea. We identify the genes that may play an important role in acclimation
to season-dependent changes of salinity. The genes were involved in processes such as osmotic adjustment, energy metabolism and photosynthesis.
2019-01-01T00:00:00Z